Translational Therapeutics Research Group
Department of Neurosurgery
Brain Tumor Treatment & Research Center
NCI-Greenebaum Comprehensive Cancer Center
University of Maryland School of Medicine - Baltimore, MD
Our Team is Committed to the Development of Novel Therapies to Conquer Devastating Diseases in the Neuroscience and Cancer Fields
The glioma cell brain slice invasion model. Human U118 glioma cells stably expressing green fluorescent protein (GFP) were delivered with a micropipette onto a mouse brain slice. Photographs were taken at the indicated times using an inverted fluorescent microscope. The white line denotes the boundary between the cell delivery site and the adjacent brain tissue. Magnification is 20X.
Nanoparticle formulations designed to minimize non-specific binding enable improved penetration and dispersion within tissues. Here, nanoparticles with very dense PEG coatings (green) are shown penetrating within brain tissue in vivo. Uncoated particles (red) with hydrophobic and negative charge surface regions display limited dispersion within brain tissue. Minimizing the non-specific bindings of therapeutic formulations is a foundational principle of our work (Nance and Woodworth et al., Science Translational Medicine, 2012; Schneider et al., Biomaterials, 2015; Wadajkar et al., Journal of Controlled Release, 2017). [Image source: Science Translational Medicine 2012; Vol. 4, Issue 149ra119.